1. Panel for Association Mapping. The association panel consists of 508 maize inbred lines, including 60 from the United States’ Germplasm Enhancement of Maize, 223 from Mexico’s International Center for Maize Improvement (CIMMYT), and 225 from China’s germplasm resources. All resources were preserved by the College of Bioscience and Biotechnology of Shenyang Agricultural University.
2. Phenotypic Data Collection. Maize brace root traits were measured during reproductive growth. Brace roots in good condition, without cracking or wilting, were chosen to facilitate subsequent correlation analysis in order to obtain more accurate data. In each row, two outermost brace roots close to the ground with mid-growing were selected for measurement. Measure the brace root diameter (BRD) using an electronic vernier caliper in the middle of the base of the brace roots (Centimeter). The brace root angle (BRA) was measured between the point of occurrence of the brace root and the main stem (degree).
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