数据控|突破是我们的每一步

标签： 数据

  • Chlorophyll content and area under the curve of the associated population

    1. An association panel comprising 378 diverse maize inbred lines from the northeast of China, the temperate region in the United States and the International Maize and Wheat Improvement Center (CIMMYT), Mexico, were used for GWAS. All materials were kept in the College of Bioscience and Biotechnology, Shenyang Agricultural University.

    genotype.hmp_下载

    2. Phenotypic Data Collection. The 378 inbred lines were grown in Fushun City, Liaoning Province, China (121°74′ E, 42°14′ N) in May 2017 (17FS) and Ledong city, Hainan Province, China (108°39′ E, 18°24′ N) in November 2017 (17LD). Fifteen plants were planted in a 2.5 m long row with 0.6 m row distance, and the planting density was approximately 45,000 plants/ha. The inbred lines were labeled before pollination for standard field management. Chlorophyll content was measured using a portable chlorophyll meter (SPAD-502, plus Konica Minolta, Tokyo, Japan), which was non-destructive, fast and cheap. SPAD-502 readings were taken from five plants per plot on five dates at a 5 d interval starting 0 d after silking. There were three measurements in the middle of the ear leaf for each plant, and the average value was used for the statistical analysis. The area under the chlorophyll content curve (AUCCC) was calculated based on SPAD-502 readings on all measure dates.

    17FS-AUCCC下载
    17FS-chlorophyll-content下载
    17LD-AUCCC下载
    17LD-chlorophyll-content下载

    short link of this page（本页链接）：https://wp.me/p80aHo-L5

  • Brace root angle and diameter of association population

    1. Panel for Association Mapping. The association panel consists of 508 maize inbred lines, including 60 from the United States’ Germplasm Enhancement of Maize, 223 from Mexico’s International Center for Maize Improvement (CIMMYT), and 225 from China’s germplasm resources. All resources were preserved by the College of Bioscience and Biotechnology of Shenyang Agricultural University.

    genotype.hmp下载（Download）

    2. Phenotypic Data Collection. Maize brace root traits were measured during reproductive growth. Brace roots in good condition, without cracking or wilting, were chosen to facilitate subsequent correlation analysis in order to obtain more accurate data. In each row, two outermost brace roots close to the ground with mid-growing were selected for measurement. Measure the brace root diameter (BRD) using an electronic vernier caliper in the middle of the base of the brace roots (Centimeter). The brace root angle (BRA) was measured between the point of occurrence of the brace root and the main stem (degree).

    phenotype下载（Download）

    Short link of this page（本页链接）: https://wp.me/p80aHo-Ed

  • 统计分析的基础——数据的产生

    数据分析是非常热门的领域，掌握数据分析方法、技术和手段，就必须要有数据。作为分析的原料，我们需要先获得数据才能进行分析。研究中，通常先要产生数据。产生数据的方法主要有观察法和实验法。观察法对被观测者的影响最小，这很重要，被观测者受到的影响越小其表现出来的结果越真实。比如，观察人眼睛的颜色，观察叶片病斑的面积等。实验法是另一种生成数据的方法，在实验中，要严格控制条件，精确测量。实验通常需要做至少两类观测，即无影响（对照）观测和有影响（处理）观测，这样才能保证没有其他因素的干扰。需要注意的是，大多数情况，处理以及观测之间都有相互作用，会影响结果的可靠性。很不幸，研究中所关注的个体都很复杂，只能降低互作，无法消除。比如植物中，纯合的10个材料，在干旱和正常条件下种植，每一株都不可能完全相同，造成这个的差异就是相互作用，到底是什么引起了互作，很难弄清，因此尽量保证每一株材料的条件相同，减少互作的影响。人类是非常复杂的生物，研究人类的难度要远远高于植物，植物在使用药物的时候通常都有效果，而人却有安慰剂效应，通过强大的心理作用起到服用药物的作用，因此，有时通过双盲实验做研究，即医生和患者都不知道使用的药物是真的药物还是糖丸儿。