玉米基因 ZmSBR1 的自然变异赋予幼苗对镰刀菌的抗性

该研究通过全基因组关联分析（GWAS）发现玉米ZmSBR1自然变异与玉米幼苗对镰刀菌（Fusarium verticillioides）的抗性显著相关。ZmSBR1通过影响植物透触发免疫和次级代谢物的合成调节玉米幼苗对病菌的抗性。研究还指出，ZmSBR1不仅在幼苗期对镰刀菌引起的茎腐病起到正面调控作用，还在成株期对茎腐病起到规避作用，为玉米抗病育种提供了新的基因标记和育种素材。

由镰刀菌（Fusarium verticillioides）引起的玉米幼苗枯萎病是一种广泛发生的玉米病害，但其抗性遗传与机制尚不清楚。在这项研究中，通过MLM和3VmrMLM进行的全基因组关联分析（GWAS）分别识别了40个和20个与幼苗枯萎病抗性相关的数量性状核酸（QTN）。这些方法分别识别了49个和36个基因。两个方法均识别出的候选基因ZmSBR1的功能验证表明，与野生型（WT）相比，接种F. verticillioides后，突变株系对幼苗枯萎病的抗性降低了0.37个等级。突变株幼苗由F. verticillioides引起的茎腐病灶长度增加了86%，而与野生型相比，在接种镰刀菌（Fusarium graminearum）后，突变植株成株的茎腐病相对长度增加了35%。转录组分析显示，与野生型相比，突变体在接种后防御相关基因的表达下调，与抗性相关的次级代谢物合成减少，病原相关分子模式（PAMP）引发的免疫响应下降，导致突变玉米幼苗的抗性降低。候选基因关联分析显示大多数玉米自交系携带易感单倍型。研究还开发了一个功能性PCR标记。结果表明，ZmSBR1在幼苗和成株生长阶段对多种镰刀菌病害提供抗性，在育种中具有重要的应用价值。

内容来自文献

Song Y, Ma P, Gao J et al. (2024) Natural variation in maize gene ZmSBR1 confers seedling resistance to Fusarium verticillioides. The Crop Journal 12(3):836-844. https://www.sciencedirect.com/science/article/pii/S221451412400093X

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TY  - JOUR
T1  - Natural variation in maize gene ZmSBR1 confers seedling resistance to Fusarium verticillioides
AU  - Song, Yunxia
AU  - Ma, Peipei
AU  - Gao, Jingyang
AU  - Dong, Chaopei
AU  - Wang, Zhao
AU  - Luan, Yifan
AU  - Chen, Jiafa
AU  - Sun, Doudou
AU  - Jing, Pei
AU  - Zhang, Xuecai
AU  - Song, Weibin
AU  - Zhou, Zijian
AU  - Wu, Jianyu
JO  - The Crop Journal
VL  - 12
IS  - 3
SP  - 836
EP  - 844
PY  - 2024
DA  - 2024/06/01/
SN  - 2214-5141
DO  - https://doi.org/10.1016/j.cj.2024.05.003
UR  - https://www.sciencedirect.com/science/article/pii/S221451412400093X
KW  - Seedling blight
KW  - Stalk rot
KW  - Multiple disease resistance
AB  - Maize seedling blight caused by Fusarium verticillioides is a widely occurring maize disease, but the genetics and mechanisms of resistance are not well understood. In this study, GWAS performed by MLM and 3VmrMLM identified 40 and 20 QTNs, associated with seedling blight resistance. These methods identified 49 and 36 genes, respectively. Functional verification of candidate gene ZmSBR1 identified by both methods showed that the resistance of a mutant line to seedling blight decreased by 0.37 grade points after inoculation with F. verticillioides, compared with the WT. The length of the stem rot lesion caused by F. verticillioides increased by 86% in mutant seedlings, and the relative length of the adult plant stalk rot increased by 35% in mutant plants compared to the wild type after inoculation with Fusarium graminearum. Transcriptome analysis showed that expression of defense-related genes after inoculation was down-regulated in the mutant compared to the wild type, synthesis of secondary metabolites associated with resistance was reduced, and the immune response triggered by PAMP decreased, resulting in decreased resistance of mutant maize seedlings. Candidate gene association analysis showed that most maize inbred lines carried the susceptible haplotype. A functional PCR marker was developed. The results demonstrated that ZmSBR1 conferred resistance to multiple Fusarium diseases at the seedling and adult growth stages and had important application value in breeding.
ER  -